AT the LAb

TestS Details

Canine Lyme Disease Diagnostics (the monitoring profile)

 
    Our Anti-Borrelia Dog (IgG) test identifies antibodies to eight antigens of B. burgdorferi at the early and later stages of infection in a single sample with 100% sensitivity. An interpretation of the results is submitted with the test report. The assay allows secure and sensitive discrimination of vaccine-derived OspA antibodies from actual infections using VlsE, OspC and other antigens to differentiate between Borrelia-specific and non-specific reactions.

Specific antigens used in this test:

    VlsE, a specific surface lipoprotein of B. burgdorferi, is known as a variable major protein-like sequence expressed (VlsE). VlsE is one of the most important antigens in Lyme disease diagnostics. Infected dogs show an early strong IgG response to VlsE.

    p100 protein, flagellin and an internal flagellin fragment of B. burgdorferi is the significant antigen for the late immune response to Borrelia. Generally, IgG antibodies against the p100 are detectible in all dogs with late Lyme borreliosis.

   OspA (p31) – outer surface lipoprotein (Osp) of B. burgdorferi. The antibodies to OspA are usually observed in vaccinated dogs. OspA is downregulated during infection. Thus, antibodies to OspA are generally undetectable after natural infection in most non-vaccinated dogs.

   OspC (p25) – is another outer surface lipoprotein (Osp) of B. burgdorferi. OspC is immunogenic during early infection and can produce protective antibody responses to B. burgdorferi infection. Antibodies to OspC can be detected after three weeks of infection. The level of antibodies to OspC decreases after seven to eleven weeks and is undetectable between four to five months after infection.

    p41 (Flagellin or Flagellar filament 41kD core protein) is a protein found in the hollow cylinder forming the filament in B. burgdorferi flagellum. The flagella play a role in the Borrelia invasion of host tissue. p41 is associated with delayed IgG response and typically detected in late Lyme borreliosis. The p41 IgG response persists with a prolonged illness.

    p39 protein, or Basic membrane protein A (BmpA), is the immunogenic cell membrane component presented on the outer surface of B. burgdorferi. BmpA is an important antigen for a B. burgdorferi infection diagnostic. IgG antibodies to p39 are frequently observed in Lyme borreliosis cases, mainly in late infections. However, these antibodies can also be detected in the early stages of Lyme disease (BmpA is expressed during the invasion of the spirochete and in the development of the arthritis of Lyme disease in dogs).

    p18 is the variable region of the Borrelia burgdorferi flagellin (an 18-kDa fragment). The IgG antibodies against the 18-kDa proteins are frequently detected in late Lyme disease infections.

    p21 protein is a member of the OspE-F gene family. Specific IgG antibodies against p21protein can be detected at either early- or late-stages of Lyme disease.

Equine Lyme Disease Diagnostics

 
    Our Anti-Borrelia ELISA Horse (IgG) test identifies equine antibodies of IgG class against Borrelia antigens in serum and plasma with 100% sensitivity. It also provides a secure confirmation of Borrelia-specific reactions. An interpretation of the results will be submitted with the test report.

Canine Ecinococcus Test

 

Equine West Nile Virus Diagnostics

 
Anti-West Nile Virus ELISA Horse (IgG) test provides semi-quantitative determination of specific antibodies of immunoglobulin class IgG against West Nile virus in equine serum or plasma with high sensitivity and specificity. An interpretation of the results will be submitted with the test report.

    The Ecinococcus test is a highly sensitive test that detects the Echinococcus-specific IgG antibodies against Echinococcus granulosus and Echinococcus multilocularis in serum and plasma of infected dogs. This test also enables differentiation between cystic and alveolar echinococcosis, caused by E. granulosus and E. multilocularis, respectively. This specific diagnostic test allows differentiating infections with E. granulosus and E. multilocularis, and detects the IgG antibodies to the following species-specific proteins:

   Em95 is E. multilocularis secreted protein, a dominant oncospheral antigen that is upregulated during oncosphere activation and is involved in cell adhesion. This is a host-protective antigen.

   Em18 is E. multilocularis antigen. Em18 is a fragment of the EM10 protein that is expressed by the larval stage of the parasite and associated with parasite invasive growth. The EM10 belongs to the ezrin-radixin-moesin family of cytoskeletal effector proteins that link to membrane-bound proteins at the cell surface and relate to parasitic metacestode proliferation and degeneration.

   EgAgB - genus-specific Echinococcus antigen is an abundant lipoprotein released by the larva of E. granulosus into the host tissues. This protein has been shown to play an important role in modulating host immune responses. It also plays a role in the parasite’s lipid metabolism.

   p7 - antigen specific for Echinococcus.

   p16/p18 - antigens specific for Echinococcus.

   p21 - antigen specific for Echinococcus and other parasites.

   p25/p26 antigens are surface proteins that protect the parasite from a harmful environment inside the midgut. These proteins are associated with parasite invasion through the plasma membrane of midgut epithelial cells at the site of parasite penetration.